secondary antibody dapi cayman chemical 14285 Search Results


peripherin  (EnCor Biotechnology)
90
EnCor Biotechnology peripherin
Peripherin, supplied by EnCor Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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anti mouse igg h l alexa fluor 594 secondary antibody  (Abcam)
99
Abcam anti mouse igg h l alexa fluor 594 secondary antibody
Anti Mouse Igg H L Alexa Fluor 594 Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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p eif2α  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc p eif2α
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
P Eif2α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/secondary+antibody+dapi+cayman+chemical+14285/Phospho-eIF2alpha+(Ser51)+XP+Rabbit+mAb/bio_rxiv__2023__12__22__572877-87-6-8
Average 96 stars, based on 1 article reviews
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bip  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc bip
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
Bip, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p acc ser79  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc p acc ser79
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
P Acc Ser79, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/secondary+antibody+dapi+cayman+chemical+14285/Phospho-Acetyl-CoA+Carboxylase+(Ser79)+Antibody/pmc10120691-233-16-19
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mouse phospho nfκb  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mouse phospho nfκb
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
Mouse Phospho Nfκb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/secondary+antibody+dapi+cayman+chemical+14285/p-NF%CE%BAB+p65+Antibody/10__3390_slash_fermentation9020167-168-11-16
Average 96 stars, based on 1 article reviews
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mouse anti neun novus biologicals  (Novus Biologicals)
91
Novus Biologicals mouse anti neun novus biologicals
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
Mouse Anti Neun Novus Biologicals, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/secondary+antibody+dapi+cayman+chemical+14285/RBFOX3%2FNeuN+Antibody+(1B7)+%5BAllophycocyanin%5D/pm36318921-878-21-23
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assays chromium single cell 30 gem  (10X Genomics)
86
10X Genomics assays chromium single cell 30 gem
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
Assays Chromium Single Cell 30 Gem, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/secondary+antibody+dapi+cayman+chemical+14285/cellranger/pm36318921-878-174-186
Average 86 stars, based on 1 article reviews
assays chromium single cell 30 gem - by Bioz Stars, 2026-11
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iwr-1-endo adooq bioscience cat# a12737  (Adooq Bioscience LLC)
90
Adooq Bioscience LLC iwr-1-endo adooq bioscience cat# a12737
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
Iwr 1 Endo Adooq Bioscience Cat# A12737, supplied by Adooq Bioscience LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnasin plus rnase inhibitor promega cat# n2615  (Promega)
90
Promega rnasin plus rnase inhibitor promega cat# n2615
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
Rnasin Plus Rnase Inhibitor Promega Cat# N2615, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a1517001 y 27632 2hcl selleckchem  (Selleck Chemicals)
96
Selleck Chemicals a1517001 y 27632 2hcl selleckchem
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
A1517001 Y 27632 2hcl Selleckchem, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paraformaldehyde santa cruz bio cat  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology paraformaldehyde santa cruz bio cat
(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of <t>eIF2α.</t> DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.
Paraformaldehyde Santa Cruz Bio Cat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of eIF2α. DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.

Journal: bioRxiv

Article Title: Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons

doi: 10.1101/2023.12.22.572877

Figure Lengend Snippet: (A) MGO stimulates the integrated stress response (ISR) and promotes the phosphorylation of eIF2α. DRF is a prodrug and is metabolized to MMF which activates the Nrf2 antioxidant response neutralizing MGO. (B) Male (n=8 per condition) and female (n=8 per condition) mice were treated with DRF (60 or 100 mg/kg) for five days. MGO (20 ng) was administered in an intraplantar injection on the third day, 1-hour after oral DRF administration for the day. Mechanical and cold sensitivity was assessed using von Frey and acetone tests, respectively. Acetone test was performed an hour after von Frey testing was complete. We observed that male (C) and female (D) mice had reduced von Frey thresholds in response to MGO injection on day 3. Daily treatment with DRF prevented MGO-induced mechanical hypersensitivity following MGO injection on day 3. (E, F) Using area under the curve of von Frey thresholds, we determined that DRF at a dose of 100 mg/kg were effective at preventing MGO-induced tactile hypersensitivity in males while both 60 mg/kg and 100 mg/kg doses were effective in females. MGO produced cold hypersensitivity in male (G) and female (H) mice which was abrogated in DRF treated mice at both doses. For (C, D, G, H) significance was calculated with repeated measures two-way ANOVA with Tukey’s multiple comparison test. *, &, # p<0.05; **, ## p<0.01; ***, &&& p<0.001; **** p<0.0001. * indicates comparison to veh-only, & indicates comparison to MGO+DRF-60, # indicates comparison to MGO+DRF-100. For (E, F) signficance was caluclated with a one-way ANOVA with Brown-Forsythe correction. * p<0.05; ** p<0.01.

Article Snippet: Antibodies used in this study were: p-eIF2α (1:500, Cell Signaling #3398), β3 tubulin (1:1000, Sigma #T8578), DAPI (1:10,000, Cayman Chemicals #14285), Alexa Fluor 488 (1:500, Life Technologies, A11034), and Alexa Fluor 555 (1:500, Life Technologies, A21428).

Techniques: Phospho-proteomics, Injection, Produced, Comparison

(A-C) Representative images of immunohistochemistry of mouse DRG neurons cultured for 24 hours and subsequently treated with vehicle-only, MGO (1µM), or MGO (1µM) plus MMF (10, 20, 50 µM). (D) We observed a significant increase in the levels of p-eIF2α when cells were treated with MGO as compared to vehicle-treated cells. MMF treatment with MGO prevented elevation of p-eIF2a particularly at 20 µM and 50 µM concentrations. Neurons were identified by their expression of beta3 tubulin. Vehicle-only (n=18), MGO (n=37), MGO+10µM MMF (n=20), MGO+20µM MMF (n=16), MGO+50µM MMF (n=32). A one-way ANOVA was used to calculate significance. *p<0.05, **p<0.01.

Journal: bioRxiv

Article Title: Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons

doi: 10.1101/2023.12.22.572877

Figure Lengend Snippet: (A-C) Representative images of immunohistochemistry of mouse DRG neurons cultured for 24 hours and subsequently treated with vehicle-only, MGO (1µM), or MGO (1µM) plus MMF (10, 20, 50 µM). (D) We observed a significant increase in the levels of p-eIF2α when cells were treated with MGO as compared to vehicle-treated cells. MMF treatment with MGO prevented elevation of p-eIF2a particularly at 20 µM and 50 µM concentrations. Neurons were identified by their expression of beta3 tubulin. Vehicle-only (n=18), MGO (n=37), MGO+10µM MMF (n=20), MGO+20µM MMF (n=16), MGO+50µM MMF (n=32). A one-way ANOVA was used to calculate significance. *p<0.05, **p<0.01.

Article Snippet: Antibodies used in this study were: p-eIF2α (1:500, Cell Signaling #3398), β3 tubulin (1:1000, Sigma #T8578), DAPI (1:10,000, Cayman Chemicals #14285), Alexa Fluor 488 (1:500, Life Technologies, A11034), and Alexa Fluor 555 (1:500, Life Technologies, A21428).

Techniques: Immunohistochemistry, Cell Culture, Expressing

(A-C) Representative images of human DRG neurons obtained from organ donors treated with vehicle, MGO (1µM), and MGO plus MMF. (D) MGO (1µM) increases p-eIF2α in human DRG neurons which is prevented with 20 µM and 50 µM MMF cotreatment. Vehicle-only (n=170), MGO (n=152), MGO+10µM MMF (n=125), MGO+20µM MMF (n=176), MGO+50µM MMF (n=111). A one-way ANOVA was used to calculate statistical significance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Journal: bioRxiv

Article Title: Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neurons

doi: 10.1101/2023.12.22.572877

Figure Lengend Snippet: (A-C) Representative images of human DRG neurons obtained from organ donors treated with vehicle, MGO (1µM), and MGO plus MMF. (D) MGO (1µM) increases p-eIF2α in human DRG neurons which is prevented with 20 µM and 50 µM MMF cotreatment. Vehicle-only (n=170), MGO (n=152), MGO+10µM MMF (n=125), MGO+20µM MMF (n=176), MGO+50µM MMF (n=111). A one-way ANOVA was used to calculate statistical significance. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: Antibodies used in this study were: p-eIF2α (1:500, Cell Signaling #3398), β3 tubulin (1:1000, Sigma #T8578), DAPI (1:10,000, Cayman Chemicals #14285), Alexa Fluor 488 (1:500, Life Technologies, A11034), and Alexa Fluor 555 (1:500, Life Technologies, A21428).

Techniques: